F/2 Nutrient Solution
Our ARS F/2 Nutrient Solution is a highly concentrated, single-part liquid formulation based on the widely renowned Guillard F/2 medium, which has been a cornerstone in microalgae culturing for decades. This versatile nutrient mix is one of the most commonly used formulas for growing marine microalgae, specifically designed for seawater strains, though we have also seen great success with various freshwater species.
Concentration and Usage:
This stock solution is provided as a 2000x concentrated solution, requiring just 0.5 mL of stock per 1 liter of water to achieve the ideal nutrient balance for your cultures. This high concentration allows for efficient storage and use, making it an excellent choice for both small and large-scale operations.
Complete Nutrient Profile:
The ARS F/2 Nutrient Solution contains all the essential macro and micronutrients required for healthy microalgal growth, including a precise blend of trace metals and vitamins. Whether you're culturing diatoms, dinoflagellates, or other marine phytoplankton, this nutrient mix provides everything your cultures need to thrive.
Concentration of Nutrients in Final Medium:
| Component |
Molar Concentration in Final Medium |
| NaH2PO4 H2O |
4.78 x 10^-5 M |
| FeCl3 6H2O |
1.54 x 10^-5 M |
| Na2EDTA 2H2O |
1.54 x 10^-5 M |
| CuSO4 5H2O |
5.19 x 10^-8 M |
| Na2MoO4 2H2O |
3.43 x 10^-8 M |
| ZnSO4 7H2O |
1.01 x 10^-7 M |
| CoCl2 6H2O |
5.54 x 10^-8 M |
| MnCl2 4H2O |
1.20 x 10^-6 M |
| Vitamins |
|
| Thiamine HCl (Vitamin B1) |
3.91 x 10^-7 M |
| Biotin (Vitamin H) |
2.71 x 10^-9 M |
| Cyanocobalamin (Vitamin B12) |
4.87 x 10^-10 M |
Guillard, R.R.L. 1975. Culture of phytoplankton for feeding marine invertebrates. pp 26- 60. In Smith W.L. and Chanley M.H (Eds.) Culture of Marine Invertebrate Animals. Plenum Press, New York, USA.
Guillard, R.R.L. and Ryther, J.H. 1962. Studies of marine planktonic diatoms. I. Cyclotella nana Hustedt and Detonula confervacea Cleve. Can. J. Microbiol. 8: 229- 239.
