Drinking water experiment #1: How much bleach is needed to kill an algae bloom?

Materials (Algae Experiment Kit)

Algae culture, Nannochloropsis or Chlorella
Secchi stick
Drippers
Test tubes or self standing tubes
Teacher supply, bleach

Methods

Record the concentration of sodium hypochlorite on the bottle of household bleach, should be between 3-6.5%.

Wear eye protection when using bleach~

Pour equal amounts of algae in to each of eight tubes

Record the secchi disk depth
Observe using all of our senses

Add bleach in exponential method (see table below), or whatever works best

bottle	mL bleach	Drops bleach
1	0	0
2	0.05	1
3	0.1	2
4	0.2	4
5	0.4	8
6	0.8	16
7	1.6	32
8	3.2	64

3. Observe for at 1 minute, 10 minutes, and 100 minutes, and 1 day

4. Results

1-minute: The algae at the higher end of the concentrations will turn white immediately after the bleach is added. This is really cool for kids to see. The algae immediately will not sink, thus the SSD will not change
10-minutes: The algae in the middle ranges will turn white, but perhaps not as completely as the higher concentrations. Again there should be no change in ssd
100 minutes: all the ‘bleaching’ should be complete. SSD may begin to change as algae sinks to the bottom. Caution should be done to prevent resuspension of the algae (dont shake it). Why… by day-1 the dead algae will sink and the SSD will increase.
1- day: the dead will sink, the live will continue to live

Algae Bleaching Experiment

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