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Our freshwater nutrient media is based on Bolds Basal Media. It should be dosed at 1mL/L, as is all of our nutrient medias (however, you can give up to 10x before it becomes toxic).
The DIY recipe for Bolds Media is here.
Bold’s Basal Media is a freshwater algae medium that is used to grow freshwater algal cultures without the need for soil-extract or vitamins (Brown et al., 1964; Nichols and Bold, 1965)
For more exciting experiments visit our YouTube page!